@article { author = {Piervandi, Z. and Khodadadi Darban, A. and Mousavi, Seyed M. and Abdollahi, M. and Asadollahfardi, Gh.R. and Akbari Noghabi, K.}, title = {A Proposed Biochemical Protocol to Isolate and Characterize Acidophilic Bacteria from Tailings Soil}, journal = {Journal of Mining and Environment}, volume = {11}, number = {4}, pages = {1157-1171}, year = {1999}, publisher = {Shahrood University of Technology}, issn = {2251-8592}, eissn = {2251-8606}, doi = {10.22044/jme.2020.10054.1942}, abstract = {Indigenous acidophilic bacteria separated from mine-waste can be used in return for the addition of the reagents like sulfuric acid. Among the tailings bacteria, Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans are of the most-studied ones for the bioleaching and bioremediation of elements. In this work, the isolation and characterization of the mentioned bacteria are studied by a proposed biochemical protocol. The sequential cultivation of the soil bacteria in a series of liquid media and solid cult}, keywords = {}, url = {https://jme.shahroodut.ac.ir/article_1902.html}, eprint = {https://jme.shahroodut.ac.ir/article_1902_06fdd3e9f15762ea4f3210ccd311d5dc.pdf} }