Document Type : Original Research Paper


1 Department of Cellular and Molecular Biology, Faculty of natural sciences and life, University of El-oued, Algeria

2 Laboratory of Biodiversity and Application of Biotechnology in the agricultural Field, Faculty of natural sciences and life, University of El-oued, Algeria

3 1. Department of Cellular and Molecular Biology, Faculty of natural sciences and life, University of El-oued, Algeria


The aim of this work is to assess the impact of red clay on the physiological and biochemical alterations in rats exposed to lead acetate. The experimental study was carried out in the laboratory on 18 male Wistar rats, which were divided into three groups of six rats in each, the first group served as control, the second group contaminated with lead, and the third group contaminated with lead and treated with red clay. Some biochemical, haematological, and oxidative stress parameters are analysed. Regarding the characterization of the clay, the results of infrared spectroscopy and X-ray spectroscopy indicate the presence of several bands indicating the mineral richness of red clay. From the analysis of our results, we observe a considerable change in the body weight, and an increase in the relative weight of liver and testis in the rats contaminated with lead compared to control. The results also show a significant decrease (p < 0.001) in the serum calcium and iron. However, serum transaminases (GPT, GOT) activities are increased in the rats contaminated with lead in comparison with control. The results obtained reveal also a decrease in the Hb, HCT, and MCV levels and in the GSH concentration and an increase in the PLT, MDA, GST, and SOD levels compared to control. Also the results clearly show alterations in the structures of the liver and testis in comparison with the controls. Treatment with red clay partially ameliorates the previous parameters, with protection and regeneration of the tissue against free radical attacks caused by lead. In conclusion, this study shows that treatment with red clay induces a positive effect against lead toxicity at molecular and tissue level.


[1]. Balali-Mood, M., Naseri, K., Tahergorabi, Z., Khazdair, M.R. and Sadeghi, M. (2021). Toxic Mechanisms of Five Heavy Metals: Mercury, Lead, Chromium, Cadmium, and Arsenic. Frontiers in pharmacology, 12, 643972.
[2]. Wani, A.L., Ara, A. and Usmani, J.A. (2015). Lead toxicity: a review. Interdisciplinary toxicology. 8 (2): 55–64.
[3]. Orr, S.E. and Bridges, C.C. (2017). Chronic Kidney Disease and Exposure to Nephrotoxic Metals. International journal of molecular sciences. 18 (5): 1039.
[4]. Derouiche, S., Ahmouda, I. and Moussaoui, R. (2021). Effectiveness of a Novel SeNPs synthetized by Aquilaria malaccensis extract compared to selenium acetate on lead-induced metabolic disorder and oxidative stress in pregnant rats. International Journal of Chemical and Biochemical Sciences. 19, 50-57.
[5]. Hammadi, N., Chouia, M. and Derouiche, S. (2021). A Study of the Relationship between Oxidative Stress and Risk of Developing Hepatocellular Carcinoma in People with Hepatitis B Infection; A Systematic Review Study." Asian Pacific Journal of Cancer Biology. (4): 316-320.
[6]. Derouiche, S., Ahmouda, I. and Moussaoui, R. (2022). Study of the toxic effect of drinking water contaminated with lead on liver and kidney functions in pregnant rats: the benefic role of aquilaria malaccensis l. spice. Malaysian Journal of Biochemistry & Molecular Biology. 25 (2): 43 - 51.
[7]. Pizzino, G., Irrera, N., Cucinotta, M., Pallio, G., Mannino, F., Arcoraci, V., Squadrito, F., Altavilla, D. and Bitto, A. (2017). Oxidative Stress: Harms and Benefits for Human Health. Oxidative medicine and cellular longevity, 2017, 8416763.
[8]. Kumar S. (2018). Occupational and Environmental Exposure to Lead and Reproductive Health Impairment: An Overview. Indian journal of occupational and environmental medicine. 22 (3): 128–137.
[9]. Külekçi,  G. (2022). Investigation of gamma ray absorption levels of composites produced from copper mine tailings, fly ash, and brick dust. Journal of Material Cycles and Waste Management. 24 (5): 1934-1947.
[10]. Derouiche, S., Kenioua, A. and Benoune, K. (2022). Characterization of natural extracted green clay and study of its effect against lead toxicity in rats. Fresenius Environmental Bulletin. 31 (11): 10934-10941.
[11]. Külekçi, G., Erçikdi, B. and Aliyazicioğlu, Ş. (2016). Effect of waste brick as mineral admixture on the mechanical performance of cemented paste backfill, IOP Conference Series: Earth and Environmental Science. 44 (4): 042039.
[12]. Da Rocha, M.C., Galdino, T., Trigueiro, P., Honorio, L.M., de Melo Barbosa, R., Carrasco, S.M. and Viseras, C., (2022). Clays as Vehicles for Drug Photostability. Pharmaceutics. 14 (4): 796.
[13]. Ríos-Reyes, C.A., Ríos-Gutiérrez, M.P. and Joya-Neira, S., 2021. The importance of minerals in medical geology: impacts of the environment on health. Archivos de Medicina (Col). 21(1): 182-208.
[14]. Massaro, M., Colletti, C.G., Lazzara, G. and Riela, S. (2018). The Use of Some Clay Minerals as Natural Resources for Drug Carrier Applications. Journal of functional biomaterials. 9 (4): 58.
[15]. Williams, L.B. and Haydel, S.E. (2010). Evaluation of the medicinal use of clay minerals as antibacterial agents. International geology review. 52 (7/8): 745–770.
[16]. García-Romero, E., Lorenzo, A., García-Vicente, A., Morales, J., García-Rivas, J. and Suárez, M. (2021). On the structural formula of smectites: a review and new data on the influence of exchangeable cations. Journal of applied crystallography. 54 (Pt 1): 251–262.
[17]. Kloprogge, J. T.T. and Hartman, H. (2022). Clays and the Origin of Life: The Experiments. Life (Basel, Switzerland). 12 (2): 259.
[18]. Dawn, L.G., Stewart, J.C. and Hugh, C.G. (2010). Role of Clay Minerals in Oil-Forming Reactions. The Journal of Physical Chemistry A. 114 (10): 3569-3575.
[19]. Yagi, K. (1976). Simple fluorimetric essay for lipoperoxide in blood plasma. Biochemical Medical. (15): 212-216.
[20]. Weckbercker, G. and Cory, J.G. (1988). Ribonucleotide reductase activity and growth of glutathione-depleted mouse leukemia L1210 cells in vitro. Cancer Letters. 40 (3): 257264.
[21]. Habig, W.H., Pabst, M.J. and Jakoby, W.B. (1974). Glutathione S-transferase. The first enzymatic step in mercapturic acid formation. Journal of Biological Chemistry, 249, 7130-7139.
[22]. Beauchamp, C. and Fridovich, I. (1971). Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry. 44 (1): 276-287.
[23]. Andala, D. and Maina, W.D. (2015). Reversal of plasticity of acid leached kaolinitic clays from Mukurwe-ini. African Journal of Pure and Applied Chemistry. 10 (4): 71-80.
[24]. Maged, A., Ismael, I.S., Kharbish, S., Sarkar, B., Peräniemi, S. and Bhatnagar, A. (2020). Enhanced interlayer trapping of Pb(II) ions within kaolinite layers: intercalation, characterization, and sorption studies. Environmental science and pollution research international, 27 (2): 1870–1887.
[25]. Miller, M.A. and Zachary, J.F. (2017). Mechanisms and Morphology of Cellular Injury, Adaptation, and Death. Pathologic Basis of Veterinary Disease, 2–43.e19.
[26]. Derouiche S. and Zeghib K. (2019). Evaluation of the Chelating Efficacy of Aquilaria malaccensis and Aristolochia longa Against Biochemical Alterations Induced by Lead Bioaccumulation in Rats. Pharmaceutical and Biosciences Journal. 7 (2): 11-15.
[27]. La-Llave-León, O., Méndez-Hernández, E.M., Castellanos-Juárez, F.X., Esquivel-Rodríguez, E., Vázquez-Alaniz, F., Sandoval-Carrillo, A., García-Vargas, G., Duarte-Sustaita, J., Candelas-Rangel, J.L. and Salas-Pacheco, J.M. (2017). Association between Blood Lead Levels and Delta-Aminolevulinic Acid Dehydratase in Pregnant Women. International journal of environmental research and public health. 14 (4): 432.
[28]. Bu-Zeinah, G. and DeSancho, M.T. (2020). Understanding Sideroblastic Anemia: An Overview of Genetics, Epidemiology, Pathophysiology and Current Therapeutic Options. Journal of blood medicine, 11, 305–318.
[29]. Türk, T., Boyraz, T. and Alp, İ. (2020). Arsenic removal from groundwater in Kutahya, Turkey, by novel calcined modified hydrotalcite, Environmental Geochemistry and Health. 42 (5): 1335-1345.
[30]. Irshad, K., Rehman, K., Akash, M. and Hussain, I. (2021). Biochemical Investigation of Therapeutic Potential of Resveratrol Against Arsenic Intoxication. Dose-response: a publication of International Hormesis Society. 19 (4): 15593258211060941.
[31]. McGill M.R. (2016). The past and present of serum aminotransferases and the future of liver injury biomarkers. EXCLI journal, 15, 817–828.
[32]. Xu, Z., Zhang, D., He, X., Huang, Y. and Shao, H. (2016). Transport of Calcium Ions into Mitochondria. Current genomics, 17(3): 215–219.
[33]. Zerzour, A., Heddig, N.E. and Derouiche, S. (2020). Analysis of Osteoporosis risk factors in Menopausal women's of Algeria population. Asian Journal of Research in Pharmacological Sciences. 10 (2):79-84.
[34]. Reddy, Y.S., Srivalliputturu, S.B. and Bharatraj, D.K. (2018). The effect of lead (Pb) exposure and iron (Fe) deficiency on intestinal lactobacilli, E. coli and yeast: A study in experimental rats. Journal of occupational health. 60 (6): 475–484.
[35]. Türk, T., Boyraz, T. and Alp, I. (2021). Fe-based layered double hydroxides for removing arsenic from water: sorption-desorption-regeneration, Journal of Water and Health, 19, 457-467.
[36]. Damato, A., Vianello, F., Novelli, E., Balzan, S., Gianesella, M., Giaretta, E. and Gabai, G. (2022). Comprehensive Review on the Interactions of Clay Minerals With Animal Physiology and Production. Frontiers in veterinary science, 9, 889612.
[37]. Kouadria, M., Djemli, S. and Tahraoui, A. (2019). The protective effect of zinc and magnesium against subchronic cadmium toxicity in wistar rats (biochemical and neurobehavioral effects) , Asian Journal of Pharmaceutical and Clinical Research. 12 (5): 1-9.
[38]. Kwon, D.H., Cha, H.J., Lee, H., Hong, S.H., Park, C., Park, S.H., Kim, G.Y., Kim, S., Kim, H.S., Hwang, H.J. and Choi, Y.H. (2019). Protective Effect of Glutathione against Oxidative Stress-induced Cytotoxicity in RAW 264.7 Macrophages through Activating the Nuclear Factor Erythroid 2-Related Factor-2/Heme Oxygenase-1 Pathway. Antioxidants (Basel, Switzerland). 8 (4): 82.
[39]. Charkiewicz, A.E. and Backstrand, J.R. (2020). Lead Toxicity and Pollution in Poland. International journal of environmental research and public health. 17 (12): 4385.
[40]. Das U.N. (2021). "Cell Membrane Theory of Senescence" and the Role of Bioactive Lipids in Aging, and Aging Associated Diseases and Their Therapeutic Implications. Biomolecules. 11 (2): 241.
[41]. Kovács, S., Kutasy, E. and Csajbók, J. (2022). The Multiple Role of Silicon Nutrition in Alleviating Environmental Stresses in Sustainable Crop Production. Plants (Basel, Switzerland). 11 (9): 1223.
[42]. Grzeszczak, K., Kwiatkowski, S. and Kosik-Bogacka, D. (2020). The Role of Fe, Zn, and Cu in Pregnancy. Biomolecules. 10 (8): 1176.