Document Type : Original Research Paper

Authors

• Z. Piervandi ¹
• A. Khodadadi Darban ¹
• Seyed M. Mousavi ²
• M. Abdollahi ¹
• Gh.R. Asadollahfardi ³
• K. Akbari Noghabi ⁴

¹ Mineral Processing Group, Department of Mining Engineering, Tarbiat Modarres University, Tehran, Iran

² Biotechnology Group, Department of Chemical Engineering, Tarbiat Modarres University, Tehran, Iran

³ Faculty of Engineering, Department of Civil Engineering, Kharazmi University, Tehran, Iran

⁴ National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

Abstract

Indigenous acidophilic bacteria separated from mine-waste can be used in return for the addition of the reagents like sulfuric acid. Among the tailings bacteria, Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans are of the most-studied ones for the bioleaching and bioremediation of elements. In this work, the isolation and characterization of the mentioned bacteria are studied by a proposed biochemical protocol. The sequential cultivation of the soil bacteria in a series of liquid media and solid cult