Document Type : Original Research Paper


1 Mineral Processing Group, Department of Mining Engineering, Tarbiat Modarres University, Tehran, Iran

2 Biotechnology Group, Department of Chemical Engineering, Tarbiat Modarres University, Tehran, Iran

3 Faculty of Engineering, Department of Civil Engineering, Kharazmi University, Tehran, Iran

4 National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran


Indigenous acidophilic bacteria separated from mine-waste can be used in return for the addition of the reagents like sulfuric acid. Among the tailings bacteria, Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans are of the most-studied ones for the bioleaching and bioremediation of elements. In this work, the isolation and characterization of the mentioned bacteria are studied by a proposed biochemical protocol. The sequential cultivation of the soil bacteria in a series of liquid media and solid cult

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